Investigation of different media content on meristem organization of raspberry (Rubus idaeus) in conditions in vitro Istraživanje medija različitog sadržaja na organiziranje meristema in vitro

Optimal conditions for in vitro multiplication and rooting of cv. Willamette were investigated. High level of proliferation and multiplication after 90 days of culture on Murashige and Skoog (MS) medium with 100 mg·l myo-inositol, 2 mg·l thiamineHCl, 1 mg·l piridoxine-HCl, 1 mg·l nicotinic acid and 30 g·l sucrose and 8 g·l agar was considered together with two concentrations of BA (0,2 and 2 mg·l). Half strength MS medium was also used. There was a significant variation of the proliferated shoots, depending on media. We got high level of rooting and we have investigated acclimatization of regenerants grown on sterile substrate in conditions of high humidity.